Composite

Part:BBa_K322125:Design

Designed by: Chris French and Richard Partridge Hicks   Group: iGEM10_Edinburgh   (2010-10-08)


Red light sensor (Cph8) and reporter system (lacZ


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 379
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note that Cph8 needs a bound chromophore (Phycocyanobilin) to be able to respond to light. This is coded for by BBa_K322122.


Source

This part was PCRed out of BBa_M30109 (which is truncated in the 2010 igem distribution).


References